Helix-Junction-Helix Motifs as Building Blocks of RNA Tertiary Structure

Project Leaders:

Hashim Al Hashimi, Rhiju Das, Dan Herschlag

Team:

This project is dedicated to achieving this level of understanding for a ubiquitous building block element of RNA tertiary structure: the helix-junction-helix (HJH) motif. Our studies are made possible by recent breakthroughs in experimental techniques for dissecting RNA structure ensembles. This study will mark the beginning of an effort aimed at comprehensively characterizing the entire HJH ‘periodic table’.

Goal:

Our aim is to resolve the conformational ensembles of helix-junction-helix (HJH) RNA motifs and higher order HJH assembly, and to understand their dependence on solution condition and potentially sequence.

Publications

Understanding nucleic acid-ion interactions

Jan Lipfert, Sebastian Doniach, Rhiju Das, Dan Herschlag

Annu Rev Biochem. 2014;83:813-41

DOI:

10.1146/annurev-biochem-060409-092720

Coarse grained models reveal essential contributions of topological constraints to the conformational free energy of RNA bulges

Anthony Mustoe, Hashim Al Hashimi, Charles L Brooks 3rd

J Phys Chem B. 2014 Mar 13

DOI:

10.1021/jp411478x

A Single-Stranded Junction Modulates Nanosecond Motional Ordering of the Substrate Recognition Duplex of a Group I Ribozyme

Phuong Nguyen, Xuesong Shi, Snorri Th. Sigurdsson, Dan Herschlag, Peter Z. Qin

Chembiochem. 2013 Sep 23;14(14):1720-3

DOI:

10.1002/cbic.201300376

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Helix-Junction-Helix Motifs as Building Blocks of RNA Tertiary Structure

Project Leaders:

Team:

Goal:

Publications

Understanding nucleic acid-ion interactions

DOI:

Coarse grained models reveal essential contributions of topological constraints to the conformational free energy of RNA bulges

DOI:

A Single-Stranded Junction Modulates Nanosecond Motional Ordering of the Substrate Recognition Duplex of a Group I Ribozyme

DOI:

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